大阪大学 石原研究室

ミトコンドリアのダイナミクスの研究をしています

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研究業績 Publication

(2020)

E. Ogasawara*, K. Nakada, N. Ishihara* 
Distal control of mitochondrial biogenesis and respiratory activity by extracellular lactate caused by large-scale deletion of mitochondrial DNA.
Pharmacol. Res. (in press)  PubMed
Available online 15 September 2020  link

A. Ota, T. Ishihara, N. Ishihara 
Mitochondrial nucleoid morphology and respiratory function are altered in Drp1-deficient HeLa cells.
J. Biochem. 167: 287-294 (2020) PubMed

O. Udagawa*, N. Ishihara*
Mitochondrial dynamics and interorganellar communication in the development and dysmorphism of mammalian oocytes.
J. Biochem. 167: 257-266 (2020) PubMed
in JB Special Issue—Dynamics Regulation of Mitochondria (Volume 167, Issue 3, March 2020), Guest edited by Naotada Ishihara and Howard Jacobs

T. Ban, N. Ishihara
Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System.
Methods Mol. Biol. 2159:115-127 (2020)  PubMed
(Book Title: Dynamin Superfamily GTPases. Methods and Protocols. 2020. Editors: Ramachandran, Rajesh (Ed.))

久保友彦、荒河匠、北崎一義、風間智彦、竹中瑞樹、坂本亘、石原直忠、中村崇裕、新倉聡、有村慎一、半田裕一、肥塚 信也
応用展開が進みつつある植物ミトコンドリア基礎研究と今後の課題
育種学研究 22 :   87-94 (2020) (in Japanese)

安田樹、石原孝也、石原直忠
ミトコンドリア構造の動的変化と疾患
遺伝子医学 MOOK 35「ミトコンドリアと病気」 286-290 (2020) (in Japanese)

(2019)

古田詩唯奈 石原直忠
ミトコンドリア構造のダイナミックな制御機構
実験医学(ミトコンドリア増刊) 37 : 1890-1895 (2019)  (in Japanese)

植田 依里 伴 匡人 石原 直忠
OPA1とカルジオリピンによるミトコンドリア内膜融合の制御
生化学 (みにれびゅう)91 : 268-271 (2019)  (in Japanese)

石原直忠
ミトコンドリア膜融合反応の試験管内再構成とその分子解析
生産と技術  71 :74-76 (2019)  (in Japanese)

(2018)

T. Ban, H. Kohno, T. Ishihara, N. Ishihara
Relationship between OPA1 and cardiolipin in mitochondrial inner-membrane fusion.
Biochim. Biophys. Acta Bioenerg. 1859: 951-957 (2018)  PubMed

Y. Miyazono, S. Hirashima, N. Ishihara, J. Kusukawa, K.I. Nakamura, K. Ohta.
Uncoupled mitochondria quickly shorten along their long axis to form indented spheroids, instead of rings, in a fission-independent manner.
Sci. Rep. 8: 350. doi: 10.1038/s41598-017-18582-6. (2018) PubMed

K. Schmitt, A. Grimm, R. Dallmann, B. Oettinghaus, L.M. Restelli, M. Witzig, N. Ishihara, K. Mihara, J.A. Ripperger, U. Albrecht, S. Frank, S.A. Brown, A. Eckert.
Circadian Control of DRP1 Activity Regulates Mitochondrial Dynamics and Bioenergetics.
Cell Metab. 27: 657-666 (2018) PubMed

E. Ueda,  N. Ishihara
Mitochondrial hyperfusion causes neuropathy in a fly model of CMT2A.
EMBO Rep. 19: e46502 (2018)  Pubmed

T. Ban, N. Ishihara
Expression and Purification of Mitochondrial Membrane Protein OPA1 for Reconstitution of Membrane Fusion. 
Silkworm Biofactory: Silk to Biology. CRC Press (2018)

花田有希、石原孝也、石原直忠
ミトコンドリアの膜動態による細胞高次機能の制御
生体の科学  69 : 581-585 (2018) (in Japanese)

小笠原絵美 宇田川理 石原直忠
ミトコンドリアの動的な変化とその分化組織における意義
日本卵子学会誌  3 :33-39 (2018) (in Japanese)

(2017)

T. Ban, T. Ishihara, H. Kohno, S. Saita, A. Ichimura, K. Maenaka, T. Oka, K. Mihara, N. Ishihara
Molecular basis of selective mitochondrial fusion by heterotypic action between OPA1 and cardiolipin.
Nature Cell Biol. 19: 856-863 (2017) PubMed

N. Ishihara, M. Maeda, T. Ban, K. Mihara
Cell-free mitochondrial fusion assay detected by specific protease reaction revealed Ca2+ as regulator of mitofusin-dependent mitochondrial fusion.
J. Biochem. 162: 287–294 (2017) PubMed

T. Ban, N. Ishihara
Expression and purification of recombinant human L-OPA1 using BmNPV bacmid-silkworm expression system.
Protoc. Exch. http://dx.doi.org/10.1038/protex.2017.053 (2017) link

H. Iihoshi, T. Ishihara, S. Kuroda, N. Ishihara*, H. Saitoh*
Aclarubicin, an anthracycline anti-cancer drug, fluorescently contrasts mitochondria and reduces the oxygen consumption rate in living human cells.
Toxicol. Lett. 227: 109-114 (2017) PubMed

H. Tamada, S. Kiryu-Seo, H. Hosokawa, K. Ohta, N. Ishihara, M. Nomura, K. Mihara, K. Nakamura, H. Kiyama
Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM.
J Comp. Neurol. 525: 2535-2548 (2017) PubMed

M. Ariyoshi, M. Katane, K. Hamase, Y. Miyoshi, M. Nakane, A. Hoshino, Y. Okawa, Y. Mita, S. Kaimoto, M. Uchihashi, K. Fukai, K. Ono, S. Tateishi, D. Hato, R. Yamanaka, S. Honda, Y. Fushimura, E. Iwai-Kanai, N. Ishihara, M. Mita, H. Homma, and S. Matoba
D-Glutamate is metabolized in the heart mitochondria.
Sci. Rep. 7:43911 (2017)  PubMed

N. Ishihara, K. Mihara
PARL paves the way to apoptosis.
Nature Cell Biol. 19: 263-265 (2017)  PubMed

石原 直忠
マイトファジーによるミトコンドリアの品質管理:どこまで明らかになったのか?
実験医学(オートファジー増刊 ) 35: 2563-2571 (2017) (in Japanese)

伴匡人、石原直忠
OPA1とカルジオリピンの一方向性の働きによるミトコンドリア融合の分子基盤の解明
実験医学 カレントトピックス 35: 3252-3255 (2017) (in Japanese)

伴 匡人、石原直忠
OPA1およびカルジオリピンの一方向性のはたらきによるミトコンドリアの選択的な融合機構
ライフサイエンス 新着論文レビュー  DOI 10.7875/first.author.2017.069 (2017) (in Japanese)  link

伴 匡人、尾上 健太、石原 直忠
ミトコンドリア形態変化の生理的意義
医学のあゆみ 260: 18-23 (2017) (in Japanese)

(2016)

S. Saita, T. Ishihara, M.Maeda, S. Iemura, T. Natsume, K. Mihara and N. Ishihara
Distinct types of protease systems are involved in homeostasis regulation of mitochondrial morphology via balanced fusion and fission.
Genes Cells 21: 408-424 (2016) PubMed

S. Kiryu-Seo, H. Tamada, Y. Kato, K. Yasuda, N. Ishihara, M. Nomura, K. Mihara, H. Kiyama
Mitochondrial fission is an acute and adaptive response in injured motor neurons.
Sci. Rep. 6:28331 (2016) PubMed

B. Oettinghaus, J. Schulz, L. Restelli, C. Savoia, A. Schmidt, K. Schmitt, A. Grimm, L. Morè, J. Hench, M. Tolnay, A. Eckert, P. D’Adamo, P. Franken, N. Ishihara, K. Mihara, J. Bischofberger, L. Scorrano S. Frank
Synaptic dysfunction, memory deficits and hippocampal atrophy due to ablation of mitochondrial fission in adult forebrain neurons.
Cell Death Differ. 23: 18-28 (2016) PubMed

太田 あずさ、伴 匡人、石原 直忠
ミトコンドリアのダイナミクスとその生理機能
脳21 19: 211-216 (2016) (in Japanese)

(2015)

T. Ishihara, R. Ban-Ishihara, M. Maeda, Y. Matsunaga, A. Ichimura, S. Kyogoku, H. Aoki, S. Katada, K. Nakada, M. Nomura, N. Mizushima, K. Mihara, and N. Ishihara
Dynamics of mtDNA nucleoids regulated by mitochondrial fission is essential for maintenance of homogeneously active mitochondria during neonatal heart development.
Mol. Cell. Biol. 35: 211–223 (2015) PubMed

R. Ban-Ishihara#, S. Tomohiro-Takamiya#, M. Tani, J. Baudier, N. Ishihara*, O. Kuge*
COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1.
FEBS Lett. 589: 3126-3132 (2015)  PubMed

T. Ishihara, H. Kohno, N. Ishihara
Physiological roles of mitochondrial fission in cultured cells and mice development.
Annals of the New York Academy of Sciences. 1350: 77-81 (2015) PubMed

K. Arasaki, H. Shimizu, H. Mogari, N. Nishida, N. Hirota, A. Furuno, Y. Kudo, M. Baba, N. Baba, J. Cheng, T. Fujimoto, N. Ishihara, C. Ortiz-Sandoval, L. Barlow, A. Raturi, N. Dohmae, Y. Wakana, H. Inoue, K. Tani, J. Dacks, T. Simmen, and M. Tagaya
A role for the ancient SNARE syntaxin 17 in regulating mitochondrial division.
Dev. Cell. 32: 304–317 (2015) PubMed

A. Jahani-Asl, E. Huang, I. Irrcher, J. Rashidian, N. Ishihara, DC. Lagace, RS. Slack, DS. Park
CDK5 phosphorylates DRP1 and drives mitochondrial defects in NMDA-induced neuronal death.
Hum. Mol. Genet. 24: 4573-4583 (2015) PubMed

L. Wang, T. Ishihara, Y. Ibayashi, K. Tatsushima, D. Setoyama, Y. Hanada, Y. Takeichi, S. Sakamoto, S. Yokota, K. Mihara, D. Kang, N. Ishihara, R. Takayanagi, M. Nomura
Disruption of mitochondrial fission in the liver protects mice from diet-induced obesity and metabolic deterioration.
Diabetologia. 58: 2371-2380 (2015) PubMed

石原 直忠
ミトコンドリア分裂の生理機能とその破綻
医学のあゆみ 245: 433-439 (2015) (in Japanese)

石原 直忠
ミトコンドリアのダイナミクスとその役割
数研出版 サイエンスネット 52: 2-5 (2015) リンク (in Japanese)

伴 匡人、後藤 雅史、石原 直忠
ミトコンドリアの融合と分裂:その意義と制御機構
化学と生物 53: 27-33 (2015) リンク (in Japanese)

宇田川 理、石原 直忠
ミトコンドリア分裂因子Drp1は細胞小器官のダイナミックな再編成を通して卵子の質を維持する
細胞工学 34: 82-83 (2015) (in Japanese)

(2014)

O. Udagawa, T. Ishihara, M. Maeda, Y. Matsunaga, S. Tsukamoto, N. Kawano, K. Miyado, H. Shitara, S. Yokota, M. Nomura, K. Mihara, N. Mizushima, and and N. Ishihara
Mitochondrial fission factor Drp1 maintains oocyte quality via dynamic rearrangement of multiple organelles.
Curr. Biol. 24: 2451-2458 (2014) PubMed

石原孝也、石原直忠
細胞と組織におけるミトコンドリアの形態制御と役割
生体の科学 
65:299-303 (2014)  (in Japanese)

(2013)

R. Ban-Ishihara, T. Ishihara, N. Sasaki, K. Mihara, and N. Ishihara
Dynamics of nucleoid structure regulated by mitochondrial fission contributes to cristae reformation and release of cytochrome c.
Proc. Natl. Acad. Sci. USA. 110:11863-11868 (2013) PubMed

K. Onoue, A. Jofuku, R. Ban-Ishihara, T. Ishihara, M. Maeda, T. Koshiba, T. Itoh, M. Fukuda, H. Otera, T. Oka, H. Takano, N. Mizushima, K. Mihara, and N. Ishihara
Fis1 acts as a mitochondrial recruitment factor for TBC1D15 that is involved in regulation of mitochondrial morphology.
J. Cell Sci. 126: 176-85 (2013)  PubMed

Y. Ohba, T. Sakuragi, E. Kage-Nakadai, N.H. Tomioka, N. Kono, R. Imae, A. Inoue, J. Aoki, N. Ishihara, T. Inoue, S. Mitani, H. Arai
Mitochondria-type GPAT is required for mitochondrial fusion.
EMBO J. 32: 1265–1279 (2013)  PubMed

H. Otera, N. Ishihara, and K. Mihara
New insights into the function and regulation of mitochondrial fission.
Biochim Biophys Acta. 1833: 1256-68 (2013)  PubMed

N. Ishihara, H. Otera, T. Oka, and K. Mihara
Regulation and physiologic function of GTPases in mitochondrial fusion and fission in mammals.
Antioxid. Redox Signal. 19: 389-399 (2013) PubMed

(2012)

S. Sekine, Y. Kanamaru, M. Koike, A. Nishihara, M. Okada, H. Kinoshita, M. Kamiyama, J. Maruyama, Y. Uchiyama, N. Ishihara, K. Takeda, H. Ichijo
Rhomboid protease PARL mediates the mitochondrial membrane potential loss-induced cleavage of PGAM5.
J. Biol. Chem. 287: 34635-34645 (2012) PubMed

A. K. G. Velikkakath, T. Nishimura, E. Oita, N. Ishihara, and N. Mizushima
Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets.
Mol. Biol. Cell. 23:896-909 (2012) PubMed

石原直忠尾上健太、北潔
ミトコンドリアの形態・機能とその動的変化
Clinical Neuroscience 30:984-987 (2012) (in Japanese)

尾上健太石原直忠
ミトコンドリアの融合と分裂
生体の科学 63:430-431 (2012) (in Japanese)

(2011)

S. R. Yoshii, C. Kishi, N. Ishihara, and N. Mizushima
Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane.
J. Biol. Chem. 286: 19630-19640 (2011) (F1000、The Best of JBC) PubMed

尾上健太前田(石原)真希石原孝也石原直忠
明らかになりつつあるミトコンドリアの融合と分裂の分子機構とその生理機能
細胞工学 30: 1147-1152 (2011)(11月号特集「オルガネラ・モデリング ベールを脱ぐ分子設計図」) (in Japanese)

石原 直忠
融合と分裂によるミトコンドリアの形態制御の分子機構と生理機能
生化学(総説) 83: 365-373 (2011) 5月号 (in Japanese)

(2010)

石原直忠
特集を読むための「基礎の基礎」(特集監修:石原直忠)
細胞工学29: 418-422 (2010) (概説、2010年5月号ミトコンドリア特集) (in Japanese)

石原直忠
ミトコンドリアの融合・分裂と哺乳動物の発生・分化
細胞工学29: 443-447 (2010) (in Japanese)

石原直忠
ミトコンドリアダイナミクス -その生理的意義とメカニズム-
医学のあゆみ 232: 659-664 (2010) (in Japanese)

石原直忠
ミトコンドリアの膜構造制御とその生理機能
生化学 2010年1月号P39-42 (in Japanese)

(2009)

N. Ishihara, M. Nomura, A. Jofuku, H. Kato, S. O. Suzuki, K. Masuda, H. Otera, Y. Nakanishi, I. Nonaka, Y-i. Goto, N. Taguchi, H. Morinaga, M. Maeda, R. Takayanagi, S. Yokota, and K. Mihara.
Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice.
Nature Cell Biol., 11:958-966 (2009) PubMed

K. Yasukawa, H. Oshiumi, M. Takeda, N. Ishihara, Y. Yanagi, T. Seya, S-i. Kawabata and T. Koshiba.
Mitofusin 2 inhibits mitochondrial antiviral signaling
Science Signal. 2: ra47 (2009) PubMed

N. Ishihara, and N. Mizushima.
A receptor for eating mitochondria.
Dev. Cell 17: 1-2 (2009) PubMed

(Before 2008, N. Ishihara)

S. Tamai, H. Iida, S. Yokota, T. Sayano, S. Kiguchiya, N. Ishihara, J. Hayashi, K. Mihara, and T. Oka.
Characterization of a mitochondrial protein LETM1 that maintains the mitochondrial tubular shapes and interacts with an AAA-ATPase BCS1L.
J. Cell Sci. 121: 2588-2600 (2008) PubMed

N. Taguchi, N. Ishihara, A. Jofuku, T. Oka, and K. Mihara.
Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission.
J. Biol. Chem. 282: 11521-11529 (2007) PubMed

Y. Eura, N. Ishihara, T. Oka, and K. Mihara.
Identification of a novel protein that regulates mitochondrial fusion by modulating mitofusin (Mfn) protein function.
J. Cell Sci. 119: 4913-4925 (2006) PubMed

N. Ishihara, Y. Fujita, T. Oka, and K. Mihara.
Regulation of mitochondrial morphology through proteolytic cleavage of OPA1.
EMBO J. 25: 2966-2977 (2006) PubMed

A. Jofuku, N. Ishihara, and K. Mihara.
Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein.
Biochem. Biophys. Res. Commun. 333:650-659 (2005) PubMed

H. Otera, S. Ohsakaya, Z. Nagaura, N. Ishihara, and K. Mihara.
Export of mitochondrial AIF in response to proapoptotic stimuli depends on processing at the intermembrane space.
EMBO J. 24: 1375-1386 (2005) PubMed

N. Ishihara, Y. Eura and K. Mihara.
Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity.
J. Cell Sci. 117: 6535-6546 (2004) PubMed

Y. Eura, N. Ishihara, S. Yokota, and K. Mihara.
Two Mitofusin proteins, mammalian homologues of FZO, with distinct functions are both required for mitochondrial fusion.
J. Biochem. 134: 333-344 (2003) JB Aword  PubMed

N. Ishihara, A. Jofuku, Y. Eura and K. Mihara.
Regulation of mitochondrial morphology by membrane potential, and DRP1-dependent division and FZO1-dependent fusion reaction in mammalian cells.
Biochem. Biophys. Res. Commun. 301: 891-898 (2003) PubMed

T. Ohsato, N. Ishihara, T. Muta, S. Umeda, S. Ikeda, K. Mihara, N. Hamasaki, and D. Kang.
Mammalian mitochondrial endonuclease G. Digestion of R-loops and localization in intermembrane space.
Eur. J. Biochem. 269: 5765-5770 (2002) PubMed

A. Kuma, N. Mizushima, N. Ishihara, and Y. Ohsumi.
Formation of the ~350 kD Apg12-Apg5-Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast.
J. Biol. Chem. 277:18619-18625 (2002) PubMed

N. Ishihara, M. Hamasaki, S. Yokota, K. Suzuki, Y. Kamada, A. Kihara, T. Yoshimori, T. Noda, and Y. Ohsumi.
Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion.
Mol. Biol. Cell 12: 3690-3702 (2001) PubMed

A. Kihara, T. Noda, N. Ishihara, and Y. Ohsumi.
Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and Carboxypeptidase Y sorting in Saccharomyces cerevisiae.
J. Cell Biol. 152:519-530 (2001) PubMed

Y. Ichimura, T. Kirisako, T. Takao, Y. Satomi, Y. Shimonishi, N. Ishihara, N. Mizushima, I. Tanida, E. Kominami, M. Ohsumi, T. Noda, and Y. Ohsumi.
A ubiquitin-like system mediates protein lipidation.
Nature 408:488-492 (2000) PubMed

T. Kirisako, M. Baba, N. Ishihara, K. Miyazawa, M. Ohsumi, T. Yoshimori, T. Noda, and Y. Ohsumi.
Formation process of autophagosome is traced with Apg8p/Aut7p in yeast.
J. Cell Biol. 147:435-446 (1999) PubMed

N. Ishihara, T. Komiya, M. Sakaguchi, A. Ito, and K. Mihara.
Characterization of the initial steps of precursor import into rat liver mitoplasts.
J. Biochem. 124:111-121 (1998a) JB Aword   PubMed

N. Ishihara, and K. Mihara.
Identification of the protein import components of the rat mitochondrial inner membrane, rTim17, rTim23 and rTim44.
J. Biochem. 123:722-732 (1998b) PubMed

N. Ishihara, S. Yamashina, M. Sakaguchi, K. Mihara, and T. Omura.
Malfolded cytochrome P-450(M1) localized in unusual membrane structures of endoplasmic reticulum in cultured animal cells.
J. Biochem. 118:397-404 (1995) PubMed

(日本語総説 2008 以前: 石原直忠) (in Japanese)

石原直忠
ミトコンドリアの融合と分裂による生命機能制御
実験医学 26:2053-2059 (2008) (in Japanese)

石原直忠、三原勝芳
ミトコンドリアの分裂・融合の制御メカニズム
細胞工学 24: 799-803 (2005) (in Japanese)

石原直忠、三原勝芳
ミトコンドリアの融合と分裂を支配する分子たち
蛋白質 核酸 酵素 50: 931-939 (2005) (in Japanese)

石原直忠、三原勝芳
ミトコンドリアの融合と分裂を制御するGTPase群
蛋白質 核酸 酵素増刊「二層膜オルガネラの遺伝学」 蛋白質 核酸 酵素 50 (14 Suppl): 1868-1871 (2005) (in Japanese)

石原直忠、三原勝芳
ミトコンドリア蛋白質の生合成と膜構造制御
炎症と免疫 13: 263-270 (2005) (in Japanese)

石原直忠、三原勝芳
ミトコンドリアのダイナミクス 融合と分裂に依存したミトコンドリアの形態調節
実験医学 21: 1712-1716 (2003) (in Japanese)

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